Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum G83 decreases concentration of D-lactic acid in the serum of DPMA mice. D-lactic acid concentration in the serum was assessed by colorimetric method. Lower D-lactic acid was observed in the TreatLG and TreatMG groups which was significant only for TreatMG relative to BlankD. All data are shown as mean ± SEM. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum G83 improves DAI score and body weight loss in DPMA mice. DSS induced colitis symptoms including weight loss, diarrhea, and rectal bleeding that were monitored by DAI score (A) . TreatMG and TreatHG groups showed a lower DAI score on day 16 (B) , while other groups had no difference compared with BlankD group. L. plantarum G83 treated mice also exhibited less weight loss than the mice in BlankD and TreatMz groups (C, D) . All data are shown as mean ± SEM. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques:

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum decreases pro-inflammatory cytokines in the serum and colon tissue of DPMA mice. In the serum, TNF-α (A) and IFN-γ (B) were significantly inhibited by L.plantarum G83 administration but less than the mesalazine treated group. IL-10 (C) was increased in the TreatMG and TreatHG groups, but not in the TreatMz and TreatLG groups. In the colon tissue, TNF-α (D) decreased after L. plantarum G83 treatment, while there was no significant difference observed in IFN-γ (E) between groups. IL-4 (F) was decreased only in the TreatMG group, other groups had no difference compared with the BlankD group. IL-10 (G) was increased only in the TreatMG group; other groups had no difference compared with the BlankD group. All data are shown as mean ± SEM. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques:

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum G83 treatment improves microbiota diversity in DPMA mice. Relative abundance of the microbiota at the family level (A) . The relative abundances of Peptostreptococcaceae , Enterobacteriaceae , and Enterococcaceae were lower in all L. plantarum G83 treatment groups compared with BlankD. The relative abundances of Muribaculaceae and Fusobacteriaceae in all treatment groups were higher than that in BlankD group. Higher relative abundance of Lactobacillaceae was observed in the TreatLG group. At the genus level (B) , the TreatLG group had the highest relative abundance of Lactobacillus in all groups. There was a higher relative abundance of Fusobacterium in L. plantarum G83 treatment groups. The relative abundance of Enterococcus in all L. plantarum G83 treatment groups was lower than that in BlankD group. However, the intestinal microbiota structure of the TreatMz group was different from that of other groups; at the genus level, it had a lower relative abundance of Lactobacillus , Fusobacterium , and Enterococcus but a higher relative abundance of Akkermansia . L. plantarum G83 treatment increased the Chao1 index in the DPMA mice (C) . The Shannon index and Simpson index were significantly higher in the TreatHG group than in the TreatLG group (D, E) . PCoA (F, G) and NMDS (H, I) analysis showed that the intestinal microbiota of all treatment groups differed from each other based on Anosim and Adonis tests ( Supplementary Table S9 ).

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques:

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum G83 increases luminal short-chain fatty acid (SCFA) concentration in DPMA mice. SCFAs—including acetic acid (A) , propionic acid (B) , butyric acid (C) , and valeric acid (valeric acid and isovaleric acid) (D) —in the colon lumen were measured by high-performance gas chromatography (HPGC). The medium dose of L. plantarum G83 increased luminal concentration of all detected SCFAs compared to the other groups. The TreatMz and the TreatLG groups had numerically increased SCFA concentrations compared to the BlankD group, but only propionic acid (B) in the TreatLG group showed significance. All data are shown as mean ± SEM, n = 4. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques: Concentration Assay, Gas Chromatography

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: Expression of TLRs and NF-κB relevant genes in DMPA mice. Gene expression in colonic tissue was determined by q-PCR. All treatment groups had no significant effect on TLR1 gene expression (A) . TLR2 was activated in all L. plantarum G83 treatment groups (B) . The TreatMz group had reduced TLRs, including TLR4 (C) and TLR5 (D) . TLR9 (E) was decreased in all treatment groups. MyD88 (F) and NF-κB (G) was increased by L. plantarum G83 treatment compared with mesalazine treatment. IKKβ (H) , TNF-α (I) , IL-1β (J) and IL-6 (K) were downregulated in all treatment groups compared with BlankD group. All data are shown as mean ± SD, n = 6. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques: Expressing, Gene Expression

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum G83 treatment was associated with altered gene expression in colon of TLR receptor negative regulators. IL1R1 (A) expression was increased in all L. plantarum G83 groups with the highest gene expression level observed in the medium dose treatment group. All treatment groups had reduced IRAK-M (B) gene expression compared to BlankD. All data are shown as mean ± SEM, n = 6. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques: Gene Expression, Expressing

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum G83 increases dephosphorylation of NF-κB-p65. NF-κB-p65 (A) and MAPK-p38 (B) phosphorylation status was assessed by Western blot. Reduced ratio of phospho-p65/p65 was seen in all three L. plantarum G83 treatment groups, which was significant for TreatMG and TreatHG. There were no significant group differences in p-MAPK-p38 compared to BlankD. All data are shown as mean ± SEM, n = 5. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques: De-Phosphorylation Assay, Phospho-proteomics, Western Blot

Journal: Frontiers in Immunology

Article Title: The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice

doi: 10.3389/fimmu.2021.747045

Figure Lengend Snippet: L. plantarum G83 treatment altered Th1/Th2 and Th1/Th17 balance in DPMA mice. The ratio of CD3+CD4+/CD3+CD8+ T cells was measured by flow cytometry in peripheral blood (A) , Peyer’s patches (B) , mesenteric lymph nodes (MLN) (C) , and spleen (D) . In peripheral blood, the CD3 + CD4 + /CD3 + CD8 + ratio was reduced in the TreatHG group compared to the other groups. In Peyer’s patches (B) , the CD3 + CD4 + /CD3 + CD8 + ratio was elevated in the TreatMG and TreatHG groups compared to the other groups. However, CD3 + CD4 + /CD3 + CD8 + T cells in the MLN were only promoted in the TreatMG group (C) . Also, the CD3 + CD4 + /CD3 + CD8 + T cells in the spleen were improved by the mesalazine, low and medium dose of L. plantarum G83 (D) . Th1/Th2 (E) cell and Th1/Th17 (F) cell were elevated by mesalazine, low and medium dose of the L. plantarum G83 treatments; the mesalazine and low dose L. plantarum G83 groups had the highest ratio. All data are shown as mean ± SEM, n = 6. Data were analyzed with one-way ANOVA (Tukey’s test). Significance is denoted by letters; if two groups have different letters, this indicates that the difference between these groups was significant with p <0.05.

Article Snippet: The L. plantarum G83 (CCTCC M2016245) was isolated from giant panda feces.

Techniques: Flow Cytometry

Journal: Food Science & Nutrition

Article Title: Identification of antibacterial substances of Lactobacillus plantarum DY‐6 for bacteriostatic action

doi: 10.1002/fsn3.1585

Figure Lengend Snippet: Bacteriostatic effect of different lactic acid bacteria on pathogenic bacteria ^a

Article Snippet: Lactobacillus plantarum DY‐6 (CCTCC2017138), L. casei DY2 (CCTCC2017303), L. rhamnose DY4 (CCTCC2017279) and Pediococcus acidilactici DY5 (CCTCC2017280) were obtained from the China Center for Type Culture Collection.

Techniques: Bacteria

Journal: Food Science & Nutrition

Article Title: Identification of antibacterial substances of Lactobacillus plantarum DY‐6 for bacteriostatic action

doi: 10.1002/fsn3.1585

Figure Lengend Snippet: Growth curve of Lactobacillus plantarum DY‐6 and changes in antibacterial activity during fermentation

Article Snippet: Lactobacillus plantarum DY‐6 (CCTCC2017138), L. casei DY2 (CCTCC2017303), L. rhamnose DY4 (CCTCC2017279) and Pediococcus acidilactici DY5 (CCTCC2017280) were obtained from the China Center for Type Culture Collection.

Techniques: Activity Assay

Journal: Foods

Article Title: Quality Improvement of Zhayu, a Fermented Fish Product in China: Effects of Inoculated Fermentation with Three Kinds of Lactic Acid Bacteria

doi: 10.3390/foods11182756

Figure Lengend Snippet: The pH values ( a ), TA contents ( b ), TVB-N contents ( c ), crude fat contents ( d ), water-soluble protein contents ( e ), and soluble solid contents ( f ) of Zhayu products prepared by spontaneous fermentation (control) and fermented with L. plantarum (L.P.), P. acidilactici (P.A.), and P. pentosaceus (P.P.) at different inoculation amounts. Different letters on the bar denote significant differences ( p < 0.05).

Article Snippet: L. plantarum (CCTCC NO. M 2012396) was obtained from the China Center for Type Culture Collection (Peking, China).

Techniques: Control

Journal: Foods

Article Title: Quality Improvement of Zhayu, a Fermented Fish Product in China: Effects of Inoculated Fermentation with Three Kinds of Lactic Acid Bacteria

doi: 10.3390/foods11182756

Figure Lengend Snippet: Textural properties of Zhayu samples prepared by spontaneous fermentation (Control) and different inoculation amounts of L. plantarum , P. acidilactici , and P. pentosaceus .

Article Snippet: L. plantarum (CCTCC NO. M 2012396) was obtained from the China Center for Type Culture Collection (Peking, China).

Techniques: Control

Journal: Foods

Article Title: Quality Improvement of Zhayu, a Fermented Fish Product in China: Effects of Inoculated Fermentation with Three Kinds of Lactic Acid Bacteria

doi: 10.3390/foods11182756

Figure Lengend Snippet: Contents (mg/100 g) and taste activity value (TAV) of free amino acids in Zhayu samples prepared by spontaneous fermentation (SF) and fermented with 109 cfu/100 g of L. plantarum (L.P.), P. acidilactici (P.A.), and P. pentosaceus (P.P.).

Article Snippet: L. plantarum (CCTCC NO. M 2012396) was obtained from the China Center for Type Culture Collection (Peking, China).

Techniques: Activity Assay, Concentration Assay

Journal: Foods

Article Title: Quality Improvement of Zhayu, a Fermented Fish Product in China: Effects of Inoculated Fermentation with Three Kinds of Lactic Acid Bacteria

doi: 10.3390/foods11182756

Figure Lengend Snippet: Relative contents (μg/kg) of aroma compounds in Zhayu samples prepared by spontaneous fermentation (SF) and fermented with 10 9 cfu/100 g of L. plantarum (L.P.), P. acidilactici (P.A.), and P. pentosaceus (P.P.).

Article Snippet: L. plantarum (CCTCC NO. M 2012396) was obtained from the China Center for Type Culture Collection (Peking, China).

Techniques: Concentration Assay